Workstation With Ergonomic Features For Universiti Kuala Lumpur Midi Classroom

@#This paper presents a design process of workstation with ergonomic features for University Kuala Lumpur MIDI Classroom. The workstation is expected to support body posture, with the aim to increase comfortability of a user usage for long periods in a class. Besides that, the workstation will ensure a conducive and responsive learning environment. Discomfort and an improper position can negatively affect overall health and productivity. A new design of workstation, which allows user to sit in ideal sitting position suggested by ergonomist and easy transition from one teaching mode to the next. The researcher has designed and developed a new workstation which has ideal ergonomic sitting working position and capable accommodate 50th percentile human size. These positions were used to evaluate the comfort of the workstation. Subjective evaluations, including comparison of the prototype and standard workstation setup, were carried out using human subjects and ergonomic principles. Result showed that the new workstation is much more comfortable, supporting the body in a balanced way. Users have the freedom to stretch and relax in different working positions before they feel any noticeable discomfort. As a result, it lets user sit for a longer period without strain, thus resulting in higher productivity and concentration in classroom.

Phytochemical and Antimicrobial Study on the Leaf Extract of Tapinanthus dodoneifolius (Van Teigh) Loranthaceae

Leaves of Tapinanthus dodoneifoliusis used as traditional medicine for the treatment of menstrual pain, wound, gonorrhea, and control of diarrhea and dysentery which was extracted by cold maceration in methanol. The concentrated methanol extract was subsequently partitioned with petroleum ether, chloroform, ethylacetate and n-butanol. Agar wellDiffusion method was used to determine the antimicrobial activities of the extracts against Staphylococcus aureaus,Bacillus subtilis, Escherichia coli, Pseudomonas aeruginosa, Klebsiela pneumonia,Salmmnella typhi, Streptococcus pyogenes, Candida krusei, Candida tropicalis,andCandida albicanat different concentrations. Antimicrobial sensitivity test indicated that,the extract inhibited the growth of all microorganisms to varying degrees except Pseudomonas aeruginosaandCandida kruseirespectively. The in vitroantimicrobial screening revealed that, the extracts exhibited diverse activities against different microbe’s concentrations ranging from 2.5mg/ml-5mg/ml. The plant extract was found to contain flavonoids, terpenoids, saponins, tannins and cardiac glycosides which may be responsible for its antimicrobial properties. The result justifies the traditional use of the plant leaf in the treatment of menstrual pain, sexual transmitted diseases, wounds and control of dysentery and diarrhea Aims: This study was aimed at investigating the antihyperglycemic effect of a combined extract of Vernonia amygdalinaand croton zabensicuscompare with a hypoglycemic drug, glibenclamide.Methodology:Twenty 20 experimental animals were used (albino rats); the rats were divided equally into four groups of five rats each; namely A (control), B (glibenclamide 10mg/kg body weight), C (synergetic treatment 1000mg/kg body weight), D (synergetic treatment 500mg/kg of body weight). Diabetes was induced intraperitoneal using Alloxan Monohydrate to all the animals and their blood glucose rise above 200mg/dl.Results:It was observed that group B and group C treated with glibenclamide (10mg/kg body weight) and synergetic aqueous extract (1000mg/kg body weight) show significantdecrease in the

Pam3CSK4 enhances adaptive immune responses to recombinant Mycobacterium bovis bacille Calmette-Guérin expressing Plasmodium falciparum C-terminus merozoite surface protein-1

Objective: To determine the effects of toll-like receptor 2 (TLR-2) agonist, Pam3CSK4, on cellular and humoral immune response against recombinant Mycobacterium bovis bacille Calmette-Guérin (rBCG) expressing the C-terminus of merozoite surface protein-1 of Plasmodium falciparum. Methods: Six groups of mice (n=6 per group) received intraperitoneal phosphate buffered saline T80 (PBS-T80), BCG or rBCG in the presence or absence of Pam3CSK4. Enzyme-linked immunosorbent assay was carried out to measure serum total IgG, IgG1, IgG2a, and IgG2b production. Spleens were also harvested and splenocytes were co-cultured with rBCG antigen for in vitro determination of IL-4 and IFN-γ via enzyme-linked immunosorbent assay. Results: The production of total IgG and the isotype IgG1, IgG2a and IgG2b was significantly higher in rBCG-immunised mice than in the BCG and PBS-T80-immunised mice, and Pam3CSK4 further enhanced their productions. A similar pattern was also observed in IFN-γ production. Moreover, there was no significant difference in IL-4 production in all groups either in the presence or absence of Pam3CSK4. Conclusions: We present evidence of the adjuvant effects of TLR-2 agonist in enhancing the production of total IgG, IgG1, IgG2a, IgG2b, as well as IFN-γ in response to rBCG. However, the presence or absence of Pam3CSK4 had no effect on IL-4 production.

Detection of interleukins-6 and 8 in saliva as potential biomarkers of oral pre-malignant lesion and oral carcinoma: a breakthrough in salivary diagnostics in Pakistan

Oral cancer is at rise in our population due to increasing use of areca nut [Betel nut] with or without tobacco. It is the second frequent malignant tumour for both the gender in Pakistan. This non-interventional case control study was carried out with the aim to explore saliva as diagnostic medium for detecting interleukins [IL] 6 and 8 as biomarkers of pre-malignant lesions [PML] and oral carcinoma. Total 105 subjects were recruited and were divided into three groups "A", "B" and "C" each comprising of 35 subjects. Group "A" comprised of cases with strong clinical evidence of oral PML. Group "B" constitute clinical and histologically proven OSCC and group "C" include disease free subjects as controls. Saliva from all the recruited subjects was procured by drooling method and stored at-20[degree sign]C before further process. All the collected samples were centrifuged at 4500 rpm for 15 minutes at 4[degree sign]C. Supernatant fluid was used in ELISA for detection and quantification of IL-6 and IL-8. Data was analysed by using Chi-square test and multivariate analysis was done by non-parametric test. P-value of 0.05 was taken as standard reference. Significant co-relation was found for qualitative salivary detection of IL-6 and IL-8 among the groups [P<0.001 and <0.0001 respectively]. Regarding quantitative salivary concentration of leukotrienes, no significant co-relation was found in levels of IL-6 among the groups while there was significant association of IL-8 levels between the groups [P<0.0001].On post Hoc multiple comparison, significant co-relation was found among oral PML group and controls [P=0.001] and OSCC group and control [P=<0.0001]. In conclusion salivary detection of IL-6 and IL-8 could be used as probable biomarker for early detection of oral PML and OSCC in etiologically distinct population of Pakistan

Salivary detection of human Papilloma virus 16 and 18 in pre-malignant and malignant lesions of oral cavity: Is it feasible in Pakistani context of Socio-Cultural Taboos?

To evaluate salivary detection of HPV-16 and 18 would be feasible and informative biomarker for oral pre-malignant and malignant lesion in our population. This non-interventional, case control study was carried out at department of E.N.T, Head and Neck Surgery, Dow University of Health Sciences, Dow Medical College and Civil Hospital Karachi, Pakistan between July 2011 to December 2012. Total of 105 cases were recruited. These were divided in three groups 'A', 'B' and 'C having 35 subjects each. Group'A' constitutes patients having strong clinical evidence of oral pre-malignant lesions [PML]. Group 'B' includes historically proven oral squamous cell carcinoma [OSCC] and Group 'C' comprised disease free subjects as controls. After taking informed consent, relevant clinical history was recorded on institutional approved performa. Saliva from all subjects was procured by standard 'drooling method'. Samples were stored at +4°C and later transferred to Laboratory to store at-20°C before further process. Samples werKfcentrifuged at 4500 rpm for 15 minutes at 4°C. Cell pellets sediments were used for identification of HPV-16 and 18 by real-time PCR method. Data was entered and analysed using SPSS version 16. P-value of 0.05 was taken as standard. In group 'A', HPV-16 was detected in 3 [8.6%] cases while HPV-18 was not detected in any of the subject. In group 'B', HPV-16 was detected in 07 [20%] while HPV-18 was found in 06 [17.1%] cases. Mixed HPV-16 and HPV-18 were found in 02 [5.7%] cases. In group 'C, HPV-16 was detected in 03 [8.6%] while HPV-18 was not detected in any of the subjects. Significant relationship was observed between the groups for HPV-18 detection [P= 0.002] while for HPV-16, no significant association was found [P= 0.245]. HPV infection for the causation of oral cancer cannot be fully established possibly due to small sample size. More over differences in genetic makeup, environment, indulgence in peculiar risk factor habits, sexual practices and difficult evaluation of the acquisition of viral load due to socio-cultural and religious restrictions could be the reason

Reported Experiences of Stalking Behavior from Patients towards Psychiatrists from the Atlantic Provinces of Canada.

Aim: A study was conducted to assess the magnitude of stalking experienced by psychiatrists, its’ effects and actions taken to ameliorate the problem. Methods: A cross sectional study to assess the magnitude of stalking the consequent effects on the psychological, social and occupational life of psychiatrists; and any preventative measure taken was conducted from July 2009-April 2010. A questionnaire was sent to 301 psychiatrists practicing in Atlantic Canada. Local Ethics approval was obtained. Results: Of the 308 psychiatrists sent surveys, 116 responded (37.6%; 67 males and 47 females). One questionnaire was invalid. (Twenty-nine (15 males and 14 females) reported experiences of stalking. Fifty-eight (50.4%) were above age 50, 49(42.6%) were between ages 35-50 and (8) 7% were younger than 35. In the study cohort, 39(33.6%) psychiatrists were in pure clinical practice, 4(3.4%) were in academic practice and 71(61.2%) had combined clinical and academic practice. Among the subspecialties, 73(62.9%) psychiatrists were practicing general adult psychiatry, 19(16.4%) were child and adolescent psychiatrists, 6(5.2%) in geriatric psychiatry, 4(3.4%) in consultationliaison, 5(4.3%) in forensic, 1(0.9%) in developmental disability and 7(6.0%) were in other psychiatric specialties. Of 115 psychiatrists, 53(46.1%) were in practice for more than 15 years, 16 (13.9%) were from 11–15 years, 29 (25.2%)-from 5 to 10 years and 17(14.8%) were in practice for less than 5 years. There was no significant differences [OR (Odds Ratio values), p-values>0.05) in reported stalked for age or length of psychiatric practice. However, there was strong association of stalking with the distress experienced by the psychiatrists (p=0.00). Conclusion: The problem of stalking does exist in the Atlantic Provinces of Canada and causes immense psychological, professional and social distress among psychiatrists who fall victim to stalking behavior. The majority of mental health professionals are unaware of any laws against stalking in Canada. There is a need for more effective education, support services and redress if we are to ameliorate the burden associated with stalking behaviours.

Molecular diagnosis of oral pre-malignant lesions and oral squamous cell carcinoma in saliva-a breakthrough in Pakistan

To evaluate salivary detection of interleukin 6 and 8 and high risk HPV-16 and 18 are informative biomarkers of Oral Pre-malignant Lesion [PML] and Oral Squamous Cell Carcinoma [OSCC] in our population. July 2011 to December 2012. Total 105 cases were included. The subjects were divided in three groups 'A', 'B' and 'C' having 35 participants each. Group 'A' comprised of patients having strong clinical evidence of oral PML. Group 'B' constitutes histologically proven OSCC and Group 'C' includes disease free subjects as controls. Relevant clinical history was recorded after informed consent on institutional approved performa. Saliva was collected as per standard drooling method'. Samples were stored at +4oC and later transferred to Dow Diagnostic, Research and Reference Laboratory to store it at -20oC before further process. Samples were subjected to centrifugation at 4500 rpm for 15 minutes at 4oC. Supernatant fluid phase was used in ELISA for detection and quantification of IL6 and IL8. . Cell pellets were used for identification of high risk HPV-16 and 18 by real-time PCR. Data was entered and analyzed on SPSS version 16. P-value of 0.05 was taken as standard reference. In group 'A', IL6 was not detected in almost all the subjects except one case. IL8 was detected in 26/35 [74.3%] subjects and not detected in 09 [25.7%] cases. In group 'B', IL6 was detected in 13 [37.1%] cases and in 22 [62.9%] cases, it cannot be detected. IL8 was detected in 33 [94.3%] and it was not detected in 02 [5.7%] subjects. It is observed that IL8 is consistently found raised in group 'A' and 'B'. In group 'C', IL6 was not detected in any of the subject while IL8 was detected in 10[28.6%] cases. Significant association was found for qualitative salivary detection of IL6 and IL8 between the groups [P= < 0.0001 and < 0.0001 respectively]. Regarding quantitative salivary concentration of IL6 and IL8, no significant co-relation was found in salivary levels of IL6 between the groups while there was significant association of salivary IL8 levels between the groups [P= <0.0001]. On post Hoc multiple comparison, significant co-relation was found in IL8 levels between oral PML group and controls [P=0.001] and OSCC group and controls [P= <0.0001]. In group 'A', HPV-16 was detected in salivary samples of 3 [8.6%] cases while HPV-18 was not detected. In group 'B', HPV-16 was detected in the salivary samples of 07 [20%] cases while HPV-18 was detected in 06 [17.1%] cases. Mixed HPV-16 and HPV-18 were found in 02 [5.7%] cases. In group 'C', HPV-16 was detected in 03[8.6%] cases while HPV-18 was not detected in any of the subjects. Significant relationship was observed between the groups for salivary HPV-18 detection [P= 0.002] while for detection of HPV-16, no significant association was found [P= 0.245]. Salivary concentration of IL6 and IL8 in oral PML and oral cancer are useful biomarkers in our population. Detection of HPV infection for the causation of oral cancer cannot be fully established possibly due to small sample size. More over different genetic makeup, environmental and geographic differences, indulgence in peculiar risk factor habits and different sexual practices compared to west due to socio-cultural and religious restrictions could be the reason

Allergic conjunctivitis at Sheikh Zayed Regional Eye Care Center, Gambia

Purpose: To assess the prevalence of allergic conjunctivitis in Gambia and to determine its epidemiologic features, seasonal variations and associated ocular and systemic conditions

Methods: Records of patients clinically diagnosed with allergic conjunctivitis between April 2007 and March 2008 were reviewed. Variables including age, sex, date of presentation, and systemic and ocular findings were recorded

Results: A total of 7,912 patients were visited within the study period, out of which 624 [7.9%] were diagnosed with allergic conjunctivitis. The disease was equally distributed in male and female subjects, with high preponderance in children [54.5%]. Most patients [60.7%] presented during the dry season as compared to the rainy season. Refractive error was the most common ocular condition associated with the condition present in 7.4% of patients while the most common systemic association was asthma, reported in 1.4% of cases

Conclusion: Allergic conjunctivitis in Gambia is more common in children than in adults and has seasonal variation with more patients presenting during dry seasons. Refractive errors are the most common ocular problem associated with the disease and asthma is a systemic association

Is lateral rhinotomy, an adequate surgical approach for juvenile naso-pharyngeal angiofibroma

The objective of the study was to determine optimal surgical approach in patients with JNA. Retrospective, descriptive. The study was carried out at the department of Ear, Nose, Throat, Head and Neck Surgery, Dow Medical College and Civil Hospital, Karachi from 1996-2007. All the young males who visited out patients department with the history of recurrent intractable epistaxis, nasal obstruction, proptosis, fullness and asymmetry of face and were diagnosed as a case of JNA on the basis of history, clinical examination and CT scan, were included in the study. All the diagnosed cases of recurrent JNA and other causes with similar symptoms were excluded. Almost all the included patients underwent digital subtraction angiography with embolization of main feeding vessel 24-48 hours before surgery. Chandler staging system was used to stage the disease. Lateral rhinotomy approach was employed in majority of the cases for exploration and removal of JNA. The out come were discussed for lateral rhinotomy as an optimal surgical approach in patients with JNA in term of ease of approach, complete removal and recurrence. Total of 53 cases of JNA were included in the study. All were young males between the age group from 8-22 years. Majority of the patients were between 13 to 18 years of age. No female patient was reported. Majority of the patients presented with triad of symptoms with recurrent epistaxis [n-53] nasal obstruction [n-53] and nasal mass [n-41]. Others presented with additional symptoms of nasal discharge [n-46], snoring [n-43] speech defect [n-32], headache [n-23], facial asymmetry [n-20], visual problems, like proptosis and diplopia [n-09] and impairment of hearing [n-19]. Diagnosis was made by clinical examination, C T scan and angiographic findings with embolization 24-48 hour prior to surgery [n-45]. Lateral rhinotomy approach for extirpation of the JNA was employed in 41 cases and Weber-Furguson approach in 07 patients. Midfacial degloving approach was used in 03 patients and in 02 cases transpalatal approach was employed where the extent of the lesion was limited to nasopharynx. Recurrence was found in 03 cases of JNA approached through lateral rhinotomy during 1-2 years follow-up. Lateral rhinotomy approach to nose and nasopharynx gives an adequate exposure in almost all the cases of JNA

Hepatitis 'B' and 'C' seropositive cases in the practice of ENT-head and neck surgery, where do we stand?

To determine the frequency of hepatitis 'B' and 'C' virus in patients admitted with pathologies of Ear, Nose, Throat or Head and Neck for surgery,: Non intervention, descriptive. Department of ENT-Head and Neck Surgery Dow University of Health Sciences and Civil Hospital Karachi, from September2006 to August 2008. Sampling was done by probability stratified technique. A total number of 832 patients admitted in department of ENT. Civil Hospital Karachi were screened by ELISA method before surgery. All the subject were unaware of their serological status regarding hepatitis B surface antigen [HBs Ag] and hepatitis C antibody [HCV Ab]. Patients admitted through emergency department, those admitted for conservative management and those under 10 years of age were not included in this study. Age, gender and risk factors like history of blood transfusion, dental treatment, any surgical intervention, home deliveries and shaving by barbers were taken as variables. Statistical analysis was done on SPSS, Out of 832 patients screened, 126 [15.14%] were seropositive out of whom 52 [41.26%] were males and 74 [58 73%] were females. HBV was positive in 31 [24.60%] [M=12; F=19] and HCV was positive in 95 [75. 39%] patients [M: 38, F: 57]. Variables did not show any statistically significant effect over the results. Universal screening rather than selective screening for HBV and HCV infection of all pre surgical cases should be exercised to prevent spread of infection among healthcare workers as well as community

Cochlear and vestibular toxicity by the use of ototoxic drugs - the regional experience

The objective of the study is to find out the common pharmacologic agents causing ototoxicity in our region with their pattern of presentation and effects on the inner ear. This study was conducted at department of Ear, Nose, Throat, Head and Neck Surgery, Civil Hospital Karachi, over a period of three years from January 1998 to December 2000. A total of 44 patients were included who presented at ENT department with the diagnosis of ototoxicity. The diagnosis was established in each case by taking detailed history, through ENT examination and related investigations. All these patients were followed up regularly for a maximum of six months. Out of44 patients, 32 were male and 12 were female patients with mean age of 42.2 years. Majority of the patients had some form of cochleotoxicity with symptoms of deafness in 95.4% and tinnitus in 36.6% of the cases. Vestibular toxicity with symptoms of vertigo and sense of imbalance were presented in 29.5% of the cases. 26 patients received only one ototoxic drug while 18 patients had received more than one ototoxic drug at one time. Gentamycin was the commonest offending agent for ototoxicity in 40.9% of the cases. In this study no patient of ototoxicity was found due to macrolide antibiotics, salicylates or any non-steroidal anti-inflammatory drugs. Sensori-neural hearing loss in majority of the patients was moderate to severe in nature

Endoscopic removal of bullet from orbital apex

Penetrating injuries of face are not uncommon. Bullets or pallets may be lodged anywhere in the cavities of skull as a result of firearm injury. Lodgment of a bullet within the orbit through nose is uncommon. An eighteen 18 years old married woman sustained a bullet injury, which entered through lateral wall of the nose and lodged at left orbital apex area. The bullet was removed endoscopically via left nostril without any damage to the eye or disturbance in vision

Histological spectrum of oral leukoplakia; a clinicopathological perspective

Histological analysis of clinically diagnosed cases of oral leukoplakia for assessment of malignant potential. Observational descriptive study. Biopsies of 113 cases of clinically established oral leukoplakia were assessed for clinical appearance and correlated with histology to determine the frequency of epithelial dysplasia and carcinoma, at Department of Pathology, Dow Medical College, Dr. Ehsanullah's laboratories Pvt. limited and Dr. Tahir laboratory, Taj Medical Complex, Hamdard University Hospital during the period of 2003-2006. The histological spectrum of 113 cases revealed hyperplastic epithelium predominantly in 85 [75.22%] cases. 14[12.38%] cases displayed mild to moderate dysplasia. Severe dysplasia I carcinoma in situ was noted in 5[4.4%] cases and microinvasive carcinoma was detected in 6[5.3%] cases. This study confirms that oral leukoplakia is a precancerous lesion and that certain clinicopathological characteristics indicate greater risk to warrant prompt biopsy examination and aggressive management

Plexiform neurofibroma of external auditory canal - a rare entity

We present a case of plexiform neurofibroma of external auditory canal [EAC] in a 43 years old male. Neurofibroma of EAC presents in rare cases. It is a benign tumor arising from the connective tissue of the nerve sheath, now thought to arise from schwann cells rather than fibroblasts. This tumor causes a fusiform enlargement of a nerve. This rare case is reported in a 43 years old male, who presented with pain in the left ear, found to have a polypoidal mass in [L] external auditory canal. Histological examination of the excised specimen revealed the features suggestive of plexiform neurofibroma of external auditory canal